Suppressing mutation-induced protein aggregation in mammalian cells by mutating residues significantly displaced upon the original mutation
نویسندگان
چکیده
منابع مشابه
Singlet oxygen induced mutation spectrum in mammalian cells.
In order to characterize the molecular nature of singlet oxygen (1O2) induced mutations in mammalian cells, a SV40-based shuttle vector (pi SVPC13) was treated with singlet oxygen arising from the thermal decomposition of the water-soluble endoperoxide of 3,3'-(1,4-naphthylidene) dipropionate (NDPO2). After the passage of damaged plasmid through monkey COS7 cells, the vector was shuffled into E...
متن کاملMutating OWLs: Semantic Mutation Testing for Ontologies
Ontologies are an essential component of semantic knowledge bases and applications, and nowadays they are used in a plethora of domains. Despite the maturity of ontology languages, support tools and engineering techniques, the testing and validation of ontologies is a field which still lacks consolidated approaches and tools. This paper attempts at partly bridging that gap, taking a first step ...
متن کاملMutation systems in cultured mammalian cells.
Experimental studies on the complex process of mutagenesis are conducted in the belief that they will increase our understanding of the effects of environmental agents on human populations. Cultured mammalian cells offer distinct advantages over other biological systems for studying this problem. Mutagenesis in cultured mammalian cells is unquestionably more relevant to man than are bacterial, ...
متن کاملThe mammalian gene mutation database.
The Mammalian Gene Mutation Database (MGMD) is a comprehensive collection of published mutation data from the open literature on mammalian cell-based gene model mutation detection systems. The database currently contains approximately 30000 comprehensively described mutant spectra records and it is maintained and up- dated on a daily basis. The major objectives of the MGMD were (i) to provide a...
متن کاملMutation rates in mammalian genomes.
Knowledge of the rate of point mutation is of fundamental importance, because mutations are a vital source of genetic novelty and a significant cause of human diseases. Currently, mutation rate is thought to vary many fold among genes within a genome and among lineages in mammals. We have conducted a computational analysis of 5,669 genes (17,208 sequences) from species representing major groups...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Engineering Journal
سال: 2014
ISSN: 1369-703X
DOI: 10.1016/j.bej.2014.08.013